Composite

Part:BBa_K283017:Design

Designed by: Shi Lei, Nelson Chu, W Ling   Group: iGEM09_HKU-HKBU   (2009-10-13)
Revision as of 18:00, 20 October 2009 by NelsonChu (Talk | contribs) (Design Notes)

prosseA-rbs-T7 RNAP (Backbone: pSB1A2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 372
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 114
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2219


Design Notes

Source

The T7polymerase, prosseA promoter is self-desgined The rbs is from the registry

References