Composite

Part:BBa_K4800055

Designed by: Yifei Wu   Group: iGEM23_NJTech-China-B   (2023-10-11)
Revision as of 07:03, 11 October 2023 by Registry (Talk | contribs)

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pRSFDuet-sfp-MmCAR-YahK

We used pACYCDuet-sfp-MmCAR-YahK as a template and amplified the PT7-YciA-Ptrc-sfp-MmCAR-Yahk fragment by PCR, and homologous recombination of PT7-YciA-Ptrc-sfp-MmCAR-Yahk with the linearized pRSFDuet vector was carried out by the In-fusion cloning method to yield plasmid of pRSFDuet-sfp-MmCAR-YahK.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4145
    Illegal XbaI site found at 1415
    Illegal PstI site found at 4580
    Illegal PstI site found at 4607
    Illegal PstI site found at 5876
    Illegal PstI site found at 5903
    Illegal PstI site found at 6296
    Illegal PstI site found at 6792
    Illegal PstI site found at 7436
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4145
    Illegal NheI site found at 7704
    Illegal PstI site found at 4580
    Illegal PstI site found at 4607
    Illegal PstI site found at 5876
    Illegal PstI site found at 5903
    Illegal PstI site found at 6296
    Illegal PstI site found at 6792
    Illegal PstI site found at 7436
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4145
    Illegal BglII site found at 5134
    Illegal BglII site found at 5760
    Illegal BglII site found at 6036
    Illegal BglII site found at 6727
    Illegal BamHI site found at 4117
    Illegal XhoI site found at 7676
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4145
    Illegal XbaI site found at 1415
    Illegal PstI site found at 4580
    Illegal PstI site found at 4607
    Illegal PstI site found at 5876
    Illegal PstI site found at 5903
    Illegal PstI site found at 6296
    Illegal PstI site found at 6792
    Illegal PstI site found at 7436
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4145
    Illegal XbaI site found at 1415
    Illegal PstI site found at 4580
    Illegal PstI site found at 4607
    Illegal PstI site found at 5876
    Illegal PstI site found at 5903
    Illegal PstI site found at 6296
    Illegal PstI site found at 6792
    Illegal PstI site found at 7436
    Illegal NgoMIV site found at 6404
    Illegal NgoMIV site found at 6466
    Illegal NgoMIV site found at 6683
    Illegal NgoMIV site found at 7130
    Illegal AgeI site found at 4310
    Illegal AgeI site found at 5321
    Illegal AgeI site found at 5618
    Illegal AgeI site found at 6503
    Illegal AgeI site found at 6512
    Illegal AgeI site found at 7187
    Illegal AgeI site found at 9049
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 9122
    Illegal SapI site found at 660


[edit]
Categories
Parameters
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