Coding

Part:BBa_K4729502

Designed by: Yasoo Morimoto   Group: iGEM23_Marburg   (2023-08-25)
Revision as of 08:59, 12 October 2023 by HannahBecker (Talk | contribs)


virG N54D CDS

Transcription factor "vir G" from the two component system regulating the virulence of Agrobacterium strains. When phosphorylated it binds to the promoter region of genes responsible for plant transformation and upregulates their transcription.

General explanation of virulence

The mechanism for virulence and plant transformation is mostly conserved between A. tumefaciens and A. rhizogenes, with high similarity in the sequences of the virulence genes and their regulation (Moriguchi et al., 2001; Zhu et al., 2000). Therefore, most of the knowledge already available for A. tumefaciens can be extrapolated when working with rhizogenes strains. In fact, the swapping of Ti-plasmids in tumefaciens strains with Ri-plasmids has created some of the most commonly used Agrobacterium rhizogenes strains, including one of the strains used by our team, Arqua1.

small description of the image
The figure illustrates the key steps of Agrobacterium-mediated insertion of a target DNA (T-DNA) into the genome of a host plant. Originally, T-DNA and virulence genes were both harboured on the same plasmid, the Ti plasmid in A. tumefaciens or the Ri plasmid in A. rhizogenes. The picture shows transformation using a binary plasmid, meaning that the vir region of the Ti plasmid is separated on a helper plasmid. Virulence is induced if either phenolic compounds are secreted by the wounded plant (dicots, 1a) or have to be added manually (monocots, 1b). After diffusing through the outer membrane, these phenolic compounds are sensed by the membrane-bound sensor kinase VirA (2). VirA in turn autophosphorylates and activates VirG (3). VirG is the master regulator of the vir operon and binds as a transcription factor to the promoters of the virulence genes. These genes are involved in the transfer of the T-DNA into the host plant's genome (step 4-5). Agrobacterium-mediated transformation can be used to insert any gene region of interest into a plant's genome.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 332
    Illegal BamHI site found at 624
    Illegal XhoI site found at 271
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None