Coding
Part:BBa_K4825046:Design
Designed by: Ni Weizhao Group: iGEM23_GreatBay-SCIE (2023-10-11)
ARO10-ADH7-ATF1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1791
Illegal EcoRI site found at 3418
Illegal SpeI site found at 742
Illegal PstI site found at 620 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1791
Illegal EcoRI site found at 3418
Illegal NheI site found at 2385
Illegal SpeI site found at 742
Illegal PstI site found at 620 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1791
Illegal EcoRI site found at 3418
Illegal BglII site found at 3105
Illegal BamHI site found at 2146
Illegal BamHI site found at 4432 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1791
Illegal EcoRI site found at 3418
Illegal SpeI site found at 742
Illegal PstI site found at 620 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1791
Illegal EcoRI site found at 3418
Illegal SpeI site found at 742
Illegal PstI site found at 620 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 3532
Illegal SapI.rc site found at 4540
Design Notes
By overexpression of ARO10 and ADH7, the supply of precursors isopenaldehyde and isopentanol is increased, and the overexpression of ATF1 directly enhances the corresponding conversion to isoamyl acetate. Therefore, The composite part increases the production of isoamyl acetate in S. cerevisiae.
Source
Saccharomyces cerevisiae