Coding

Part:BBa_K4825026:Design

Designed by: Cai Luxi   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 13:29, 11 October 2023 by Registry (Talk | contribs)

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XylR-NLS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 235
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LEU2 is used as the yeast auxotrophic marker in this part, instead of normally URA3, which allows the co-transformation of the plasmid of this part and the plasmid of xylose promoters.


Source

Bacillus licheniformis

References