Coding

Part:BBa_K4825024:Design

Designed by: Cai Luxi   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 13:25, 11 October 2023 by Registry (Talk | contribs)

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pXyl


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

"1. T20N2 spacer before the first xylose operator and T24N11 spacer between the two xylose operators contain mutations to suppress recombination;

2. Transcription start site (TSS) is connected with a 10bp Kozak sequence to increase the efficiency of translation;

3. The TATA box is placed 4bp down away from T20N2 spacer to result in a strong promoter that is independent on the carbon sources for cellular growth.

4. Red fluorescence protein (RFP) is attached after the promoter to test the functionality of the promoter. "


Source

Synthesized

References