Plasmid

Part:BBa_K4815017:Design

Designed by: Chen Xi   Group: iGEM23_NJU-China   (2023-10-12)
Revision as of 07:14, 12 October 2023 by Registry (Talk | contribs)

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pDualPH7-pDual mcherry-yeGFP High 7


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 3677
    Illegal SpeI site found at 1351
    Illegal PstI site found at 991
    Illegal PstI site found at 2232
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 396
    Illegal NheI site found at 3443
    Illegal SpeI site found at 1351
    Illegal PstI site found at 991
    Illegal PstI site found at 2232
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 396
    Illegal BglII site found at 1963
    Illegal BamHI site found at 3640
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 3677
    Illegal SpeI site found at 1351
    Illegal PstI site found at 991
    Illegal PstI site found at 2232
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 3677
    Illegal SpeI site found at 1351
    Illegal PstI site found at 991
    Illegal PstI site found at 2232
    Illegal NgoMIV site found at 1862
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2922
    Illegal BsaI.rc site found at 4317
    Illegal SapI site found at 4808
    Illegal SapI site found at 5408
    Illegal SapI.rc site found at 2769


Design Notes

1


Source

1

References