Part:BBa_K4759017
Nus
Recombinant protein expression plays an important role in promoting the development of modern biological technology. By using the host to achieve exogenous protein expression, it provides a powerful tool for studying the structure and function of some important proteins, especially the human source proteins. In order to enable subsequent structure-function studies, the obtained recombinant proteins must be soluble, stable, properly folded as well as biologically active. However, in practical studies, these often become obstacles to obtaining an ideal recombinant protein. The solubility of the recombinant protein expression became the primary addressed problem.Because NusA has the biological activity to promote DNA transcription and slow translation, the expressed protein has more time to fold, allowing the recombinant protein to be stable and active. Even without removing the NusA tag, the fusion protein can still be active.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1371
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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