Coding

Part:BBa_K4825004:Design

Designed by: Cai Luxi   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 12:23, 11 October 2023 by Registry (Talk | contribs)

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Cry1518-35


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 132
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

"1. Codons are optimized for E.coli based when it is expressed in E.coli, and optimized for S.cerevisiae based when it is expressed in S.cerevisiae. 2. 6x His tag (HHHHHH) is added to either the N-terminal or C-terminal of the coding sequence for Ni-NTA purification."


Source

Bacillus thuringiensis

References