Coding

Part:BBa_K4825003:Design

Designed by: Ni Weizhao   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 12:17, 11 October 2023 by Registry (Talk | contribs)

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PS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 223
    Illegal BamHI site found at 1060
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 695


Design Notes

"1. The 51-position prolinate site at the N terminal of PtPS was truncated to increase the efficiency of pinene production. 2. PS is linked to ERG20ww with a flexible linker (GGGGSGGGGS) to increase the production of pinene from GPP, and reduce the GPP allocated to produce FPP to increase the yield of target product."


Source

Pinus taeda

References