Coding
Part:BBa_K4765025
Designed by: Siliang Zhan Group: iGEM23_Fudan (2023-10-01)
Rv Dsup codon optimized
Introduction
Rv Dsup is a damage suppressor protein from the Tardigrade Species Ramazzottius Varieornatus [1] . This is a nucleosome-binding protein that protects chromatin from hydroxyl radicals[2] , thereby inhibiting DNA molecule breakage. This protein can reduce X-ray-induced damage in human cells by 40%.
Usage and Biology
We optimized the codons of wildtype Rv Dsup for E. coli K12 and heterologously expressed it in E. coli to enhance its survival rate under UV radiation.
Characterization
Sequencing map
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| Figure1 Sequencing map of Rv Dsup Sequencing starts from the T7 terminator, with the primer 5-GCTAGTTATTGCTCAGCGG-3. |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 169
Illegal AgeI site found at 240 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 166
Illegal SapI.rc site found at 895
Reference
- ↑ Hashimoto, T., Horikawa, D. D., Saito, Y., Kuwahara, H., Kozuka-Hata, H., Shin-I, T., Minakuchi, Y., Ohishi, K., Motoyama, A., Aizu, T., Enomoto, A., Kondo, K., Tanaka, S., Hara, Y., Koshikawa, S., Sagara, H., Miura, T., Yokobori, S. I., Miyagawa, K., Suzuki, Y., … Kunieda, T. (2016). Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Nature communications, 7, 12808. https://doi.org/10.1038/ncomms12808
- ↑ Chavez, C., Cruz-Becerra, G., Fei, J., Kassavetis, G. A., & Kadonaga, J. T. (2019). The tardigrade damage suppressor protein binds to nucleosomes and protects DNA from hydroxyl radicals. eLife, 8, e47682. https://doi.org/10.7554/eLife.47682
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Categories
Parameters
//cds
| protein |