Device

Part:BBa_K4646018

Designed by: Alexander Diefes   Group: iGEM23_Duke   (2023-10-10)
Revision as of 07:03, 12 October 2023 by Rk320 (Talk | contribs)


EF1alpha _Kozak_IL-2

This construct is driven by a strong EF1alpha promoter with enhancer. It also includes a strong Kozak. It can be used to secrete a protein of interest using the IL-2 signal peptide in mammalian systems.

The Duke 2023 iGEM team used the signal peptide IL-2 as part of our study to assess the secretion efficiencies of different combinations of signal peptides, promoters, and cargos. Compared to the negative control signal peptide HA, IL-2 showed relatively high levels of secretion as shown by low levels of YFP fluorescence inside of the cells. Based on this study, we found that IL-2 has relatively high secretion efficiency when matched with a strong promoter such as CMV or EF1α.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 643
    Illegal XhoI site found at 1042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 777
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None