Coding

Part:BBa_K4765012

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-08-07)
Revision as of 05:49, 11 October 2023 by Siliang Zhan (Talk | contribs) (→‎Introduction)


MysC codon optimized contributed by Fudan iGEM 2023

Introduction

An ATP-grasp enzyme MysC converts 4-DG to mycosporineglysine (MG) by introducing L-Glycine to 4-DG at C3[1].

contributed by Fudan iGEM 2023
Figure 1. The biosynthetic pathway of shinorine, porphyra-334, palythine-Ser, and palythine-Thr

Usage and Biology

We performed codon optimization on BBa_K4273008(NpR5598) specifically for the Escherichia coli K12 strain, resulting in the creation of this part. The enzyme MysC catalyzes the third reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 736


References

  1. ↑ Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. The Journal of Organic Chemistry, 86(16), 11160–11168.
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Categories
//cds/enzyme
Parameters
protein