Coding

Part:BBa_K4765010

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-08-07)
Revision as of 06:58, 12 October 2023 by Zhenmaoye (Talk | contribs) (→‎Usage and Biology)


MysA codon optimized

contributed by Fudan iGEM 2023

Introduction

MysA encodes a dimethyl 4-degadusol synthase (DDGS) that converts Sedoheptulose 7-phosphate (S7P) into Demethyl 4-deoxygadusol (DDG)[1].

contributed by Fudan iGEM 2023
Figure 1. The biosynthetic pathway of shinorine, porphyra-334, palythine-Ser, and palythine-Thr

Usage and Biology

We performed codon optimization on BBa_K4273004(NpR5600)specifically for the Escherichia coli K12 strain, resulting in the creation of this part. The enzyme MysA catalyzes the initial reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within E. coli.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1198


References

  1. ↑ Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. The Journal of Organic Chemistry, 86(16), 11160–11168.
[edit]
Categories
//cds/enzyme
Parameters
protein