Device

Part:BBa_K4646018

Designed by: Alexander Diefes   Group: iGEM23_Duke   (2023-10-10)
Revision as of 02:44, 11 October 2023 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


EF1alpha _Kozak_IL-2

This construct is driven by a strong EF1alpha promoter with enhancer. It also includes a strong Kozak. It can be used to secrete a protein of interest using the IL-2 signal peptide in mammalian systems.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 643
    Illegal XhoI site found at 1042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 777
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None