Part:BBa_K4989009
GFP
Green Fluorescent Protein (GFP) is a naturally occurring protein that exhibits remarkable fluorescence when exposed to ultraviolet or blue light. The fluorescence of GFP arises from a chemical reaction within the protein. When exposed to UV or blue light, GFP absorbs photons and undergoes a process called fluorescence, where it emits green light. The chromophore, a small molecular group within the protein, is responsible for this fluorescence.GFP revolutionized cell and molecular biology by allowing scientists to visualize and track specific proteins or cells within living organisms. It has been used to study processes such as gene expression, protein localization, cell migration, and cell division. GFP fusions are created by attaching the GFP gene to the gene of interest, enabling researchers to monitor its expression and localization in real time. Also,It is commonly used as a reporter gene in molecular biology to assess the activity of promoters or the effectiveness of gene expression. By fusing the GFP gene to a promoter of interest, researchers can easily monitor when and where a gene is active. GFP-tagged proteins have been instrumental in studying protein localization within cells and tracking their movement. This has provided insights into organelle dynamics, vesicle transport, and the functioning of cellular structures.In genetic studies, GFP has been used to label specific cells or tissues, making it possible to trace the development of embryos, observe the migration of cells, or identify specific cell types. It has played a crucial role in understanding developmental biology and genetics.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |