Part:BBa_K4765118

ribozyme connected: MysABCDH

Introduction

Mycosporine-like amino acids (MAAs), act as a sunscreen for the biofilm. This composite part contains five enyzmes in the MAA biosynthesis pathway. All the enzymes are constructed into ribozyme-assisted polycistronic co-expression system：pRAP.

Figure 1. The biosynthetic pathway of shinorine, porphyra-334, palythine-Ser, and palythine-Thr

Usage and Biology

Biosynthetic route of MAA initiates with the generation of 4-deoxygadusol (4-DG) from sedoheptulose 7-phosphate, an intermediate within the pentose phosphate pathway. This process is catalyzed by two enzymes: a dimethyl 4-degadusol synthase (DDGS; MysA) and an Omethyltrans-ferase (O-MT; MysB). Subsequently, 4-DG undergoes a transformation into mycosporine-glycine(MG) through an ATP-grasp enzyme MysC, which introduces an amino acid moiety, primarily L-Gly. MAA analogues such as shinorine or porphyra-334 are further derived from MG by the D-Ala-D-Ala ligase-like enzyme MysD. In the final step, the biosynthesis is completed with a nonheme iron-(II)- and 2oxoglutarate-dependent (Fe/2OG) oxygenase MysH, leading to the production of palythines.

Characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1272
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1064
  Illegal BsaI.rc site found at 2666
  Illegal BsaI.rc site found at 3785
  Illegal BsaI.rc site found at 5714