Coding
Part:BBa_K4785005:Design
Designed by: ZhengLin Li Group: iGEM23_SUSTech-CHINA (2023-10-10)
high-mobility group box 1 (hmgb1)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 73
Design Notes
The protein expressed by this sequence is a nuclear protein, and the signal peptide sequence needs to be changed if it is expressed in the eukaryotic system or secreted out of the cell.
Figure 1: To purify the target proteins, we construct five plasmids: pET28a-6×His-HMGB1_FL, pET28a-6×His-HMGB1_AB, pET28a-6×His-HMGB1_A, pET28a-6×His-HMGB1_B. After sequencing, we determine that the plasmid was correctly sequenced and ready for use.
HMGB1_A and HMGB1_B appear to be more highly expressed in E. coli, presumably due to their shortened sequence lengths and reduced number of modifications, as determined experimentally.
Source
Organism: Homo sapiens