Regulatory

Part:BBa_K4788004:Design

Designed by: Zhaocheng Li   Group: iGEM23_LZU-CHINA   (2023-10-09)
Revision as of 01:55, 12 October 2023 by Zcli lzu (Talk | contribs) (→‎References)


FadO operator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

it codes FadR, it is a transcription factor that can bind to the FadBA promoter, inhibit initiation, and be released by oleoyl CoA metabolized from oleic acid.


Source

We synthesize this gene.

References

Ma Y, Zheng X, Lin Y, Zhang L, Yuan Y, Wang H, Winterburn J, Wu F, Wu Q, Ye JW, Chen GQ. Engineering an oleic acid-induced system for Halomonas, E. coli and Pseudomonas. Metab Eng. 2022 Jul;72:325-336. doi: 10.1016/j.ymben.2022.04.003. Epub 2022 May 2. PMID: 35513297.