Part:BBa_K5009001

High-efficiency TPH originates from Zebrafish

Usage

5-Hydroxytryptamine (5-HT), also known as serotonin, often referred to as the "feel-good" neurotransmitter, is involved in regulating mood, sleep, appetite, and a range of other physiological functions. Imbalances in serotonin levels, particularly a deficiency, are implicated in the pathophysiology of depression.

Therefore, we hope to produce serotonin through synthetic biology methods to treat depression. Through literature research and an analysis of previous team projects, we have found that expressing serotonin using symbiotic bacteria is a promising approach. The rate-limiting step in the biosynthesis pathway of serotonin involves tryptophan hydroxylase 1 (TPH1) catalyzing tryptophan to form 5-hydroxytryptophan, which is then converted to serotonin by tryptophan decarboxylase (TDC). For more project background visit: https://2023.igem.wiki/squirrel-chn-ii/description

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 610
  Illegal XbaI site found at 1423
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 610
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 610
  Illegal BglII site found at 299
  Illegal BglII site found at 498
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 610
  Illegal XbaI site found at 1423
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 610
  Illegal XbaI site found at 1423
  
• 1000
  COMPATIBLE WITH RFC[1000]