Coding

Part:BBa_K4815021:Design

Designed by: Chen Xi   Group: iGEM23_NJU-China   (2023-10-11)
Revision as of 01:16, 12 October 2023 by ChenXi (Talk | contribs) (→‎Design Notes)


LTB-eGFP-fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 298


Design Notes

After testing our model by the dual fluorescence, to furtherly prove our learning model’s ability, we engineered the promoter sequence of S. cerevisiae to produce E. coli heat-labile enterotoxin subunit B (LTB). Our goal is using our AI to tackle the issue of the low LTB production yield. By inserting the synthesized efficient high expression or the low expression promoters, it can generate the expression rate of the LTB which can be detected by the GFP. The western blot and the RT-PCR were adopted to test.

Source

1

References