Composite

Part:BBa_K283011

Designed by: Shi Lei, W Ling , Nelson Chu   Group: iGEM09_HKU-HKBU   (2009-10-13)
Revision as of 08:29, 13 October 2009 by Registry (Talk | contribs)

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pTet-rbs-lpp_omp(46-159)_streptavidin_Histag

The fusion protein omp(46-159)streptavidin(WT) with a 6-histidine tail is linked after a pTet promoter. The fusion protein coding region does not contain any terminator. The omp domain directs the expression of the fusion protein on the surface membrane

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 565
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 607
    Illegal AgeI site found at 658
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/generator/prc
//regulation/negative
Parameters
None