Coding

Part:BBa_K4989006:Design

Designed by: Athanasia Arampatzi   Group: iGEM23_Thrace   (2023-10-11)
Revision as of 01:00, 12 October 2023 by Atharab (Talk | contribs) (→‎References)


Butyryl-CoA:Acetyl-CoA transferase (ButCoaT)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 714
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 888
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence that we designed has a start and ending codon and a single open reading frame (ORF). Also, the sequence is expected to be expressed in our hosts Lactobacillus rhamnosus GG, Lactobacillus plantarum LP-01, Lactobacillus acidophilus DSM 20079 and E.coli K-12 and thus was codon-optimized.


Source

Our part was synthetically produced but the sequence comes from Roseburia intestinalis L1-82.

References

[1]“Roseburia Intestinalis L1-82 Chromosome 1, Complete Sequence.” NCBI Nucleotide, Mar. 2023, www.ncbi.nlm.nih.gov/nuccore/NZ_LR027880.1?report=genbank&from=430426&to=431769&strand=true. Accessed 12 Oct. 2023.
[2]Petra Louis, Sheila I. McCrae, Cédric Charrier, Harry J. Flint, Organization of butyrate synthetic genes in human colonic bacteria: phylogenetic conservation and horizontal gene transfer, FEMS Microbiology Letters, Volume 269, Issue 2, April 2007, Pages 240–247,https://doi.org/10.1111/j.1574-6968.2006.00629.x