Coding

Part:BBa_K4364001:Experience

Designed by: Slavil Peykov   Group: iGEM22_Bulgaria   (2022-10-12)
Revision as of 00:16, 12 October 2023 by Slavil (Talk | contribs) (→‎User review of iGEM Bulgaria 2023)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4364001

This part was used to generate constructs with constitutive expression that were next used for the CADABRA cocktail. See our Wiki for more details. [http://2022.igem.org/Team:Bulgaria]

User Reviews

UNIQb9996f9908b1e3ea-partinfo-00000000-QINU UNIQb9996f9908b1e3ea-partinfo-00000001-QINU

User review of iGEM Bulgaria 2023

We used this part in combination with BBa_K608003 to create an expression construct that produces VIM-2 MBL. We proved that the resulting construct was functional and we used the secreted MBL enzyme to treat ampicillin contaminations in water samples (see https://2023.igem.wiki/bulgaria/engineering).

File:Https://static.igem.wiki/teams/4855/wiki/amp-test.png

Ampicillin degradation was observed but the NDM-2&5-based version was several times more efficient and we recommend its usage instead of this part.