Translational_Unit

Part:BBa_K4855004

Designed by: Slavil Peykov   Group: iGEM23_Bulgaria   (2023-10-11)
Revision as of 23:33, 11 October 2023 by Registry (Talk | contribs)

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J23101 - T7 - medium RBS - amilGFP CDS

This composite part comprises of J23101 - T7 - medium RBS - amilGFP. It was conceptualized by the iGEM Bulgaria 2023 team as a construct intended to test the efficiency of an in vivo T7-driven mutagenesis system. It was cloned in the low copy number SC101 ori-based vector pSB4K5 and used with the dual gene-specific mutator system capable of introducing transition mutations at consistent frequencies within a target sequence in vivo that was available for ordering from Addgene. (Seo D, Koh B, Eom GE, Kim HW, Kim S. A dual gene-specific mutator system installs all transition mutations at similar frequencies in vivo. Nucleic Acids Res. 2023 Jun 9;51(10):e59. doi: 10.1093/nar/gkad266. PMID: 37070179; PMCID: PMC10250238.).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None