Coding

Part:BBa_K283007:Design

Designed by: Liu Chenli   Group: iGEM09_HKU-HKBU   (2009-10-20)
Revision as of 10:53, 20 October 2009 by Registry (Talk | contribs)

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T7 RNA polymerase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1693


Design Notes

Referenc:Polar localization of the autotransporter family of large bacterial virulence proteins.Journal of Bacteriology, 2006vol.188(no.13)



Source

Pathogenic Escherichia coli strain 2787 (O126:H27) The genes encoding AIDA (adhesin involved in diffuse adherence) are located on two open reading frames, orfA and orfB. The latter codes for the AIDA autotransporter system, which is synthesized as a pre-pro-protein that is C-terminally processed to generate the mature adhesin AIDA-I and the outer membrane integrated AIDAC, which functions as the translocator for AIDA-I. orfA codes for a 45-kDa cytoplasmic protein which is required to modify AIDA-I such that it adheres to target cells. Although the AIDA system has first been characterized in a clinical E. coli isolate responsible for infant diarrhea, AIDA genes have been detected only in about 2 to 4% of human isolates . Thus, an animal reservoir for AIDA may exist.

References