Composite

Part:BBa_K4619010:Design

Designed by: KaMing Li   Group: iGEM23_UCAS-China   (2023-10-09)
Revision as of 17:06, 9 October 2023 by Registry (Talk | contribs)

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PobR-Threshold Guard Switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 462
    Illegal AgeI site found at 154
    Illegal AgeI site found at 675
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 595
    Illegal SapI.rc site found at 1573


Design Notes

Regarding the original digitizer[1], we eliminate the XylS system, which includes the XylS protein and its corresponding promoter. We then combine these two sections and anticipate that the new chimeric digitizer will function properly. We use GFP to test our new chimeric digitizer. If it functions well, we will replace GFP with luxI to improve our bio-sensor.



Source

[1] Calles B , Goni-Moreno A , Lorenzo V D .Digitalizing heterologous gene expression in Gram-negative bacteria with a portable on/off module[J]. 2019.DOI:10.1101/783506.

References