Signalling

Part:BBa_F1610

Designed by: Caitlin Conboy and Jennifer Braff   Group: Antiquity   (2003-12-04)
Revision as of 09:37, 20 October 2009 by Koi (Talk | contribs) (Notice)

3OC6HSL Sender Device

This device accepts PoPS as input and produces the LuxI enzyme. This enzyme produces 3OC6HSL.

Notice

With a VF2-VR PCR length compraison, I found that it is empty on this plasmid sent in iGEM2007 plates. --marion 07:12, 22 March 2008 (EDT)


While present on a gel after running PCR on both 2007 and 2008 plates, the DNA appears to only be ~600bp long which conflicts with the part description. --robere 27 Jun 2008
See gel image


We use this part on iGEM2009 plate. While present on a gel after cutting the miniprep DNA extraction by EcoRI and SpeI, the restriction fragment appears to about 3000 bp and 800 bp . BBa_F1610 on iGEM2009 plate is therefore correct.--Koi 20 Oct 2009 (Tokyo_Tech) See gel|image

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 655
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/signalling/sender
//function/cellsignalling
Parameters
control
device_typeSender
family
latency
output
proteinsLuxI
signalling_molecule3OC6HSL