Composite

Part:BBa_K4604025

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-10)
Revision as of 22:54, 10 October 2023 by Registry (Talk | contribs)

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piG_23b (tetR_riboRBS_mazF)

This BioBrick consists of the tetR repressor, the RBS taken from the K12 riboswitch (BBa_K4604031), the tet promoter, the modified T7 RBS, mazF and the rrnB terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 701
    Illegal XbaI site found at 616
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 701
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 701
    Illegal BglII site found at 710
    Illegal BamHI site found at 1153
    Illegal XhoI site found at 1162
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 701
    Illegal XbaI site found at 616
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 701
    Illegal XbaI site found at 616
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None