![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4604025
Designed by: Hannah Swientek Group: iGEM23_Freiburg (2023-10-10)
piG_23b (tetR_riboRBS_mazF)
This BioBrick consists of the tetR repressor, the RBS taken from the K12 riboswitch (BBa_K4604031), the tet promoter, the modified T7 RBS, mazF and the rrnB terminator.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 701
Illegal XbaI site found at 616 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 701
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 701
Illegal BglII site found at 710
Illegal BamHI site found at 1153
Illegal XhoI site found at 1162 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 701
Illegal XbaI site found at 616 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 701
Illegal XbaI site found at 616 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |