Regulatory

Part:BBa_K4779005

Designed by: Ziqi Mi   Group: iGEM23_Nanjing-BioXstem   (2023-09-27)
Revision as of 15:06, 27 September 2023 by Registry (Talk | contribs)

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UAScit-prm1

UAScit-prm1 is a new synthetic hybrid promoter that improves the basal activity and induced expression level of the prm1 promoter. It consists of two tandem modules, the enhancer element UAScit and the prm1 promoter, where UAScit is a constitutive promoter. The fluorescence intensity of the engineered yeast strain BY4741-pRS415-UAScit-prm1-GFP-CYC1 was assessed using flow cytometry, and the data were analyzed with FlowJo and GraphPad Prism software. The findings revealed a 13.2% increase in basal activity and a 76.9% increase in induced expression levels compared to the prm1 promoter. This demonstrates that the constitutive enhancer element UAScit enhances both basal and induced expression levels, regardless of whether it is placed in front of inducible or constitutive promoters.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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