Coding

Part:BBa_K2619001

Designed by: shiyu liu   Group: iGEM18_XJTLU-CHINA   (2018-09-07)
Revision as of 07:38, 7 September 2018 by Registry (Talk | contribs)

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syndecan 4/SDC4/SYND4, syndecan-4/syntenin pathway

SDC4 is involved in syndecan-4/syntenin pathway. The formation of exosomes is controled by syndecan heparan sulphate proteoglycans and their cytoplasmic adaptor syntenin, and it is due to interaction of sydecan`syntenin complex with Alix, an ESCRT (endosomal-sorting complex required for transport) III-binding protein. Syndecan-4 supports budding of endosomal membranes to form multivesicular bodies by interacts direactly with ALIX through LYPX(n)L motifs (Baietti et al., 2012). The activation Rac1 and RhoG requires interactions of syndecan-4 and its cytoplasmic partners synectin and syntenin, and this activation will further regulate exosome biosynthesis. therefore we used this gene to increase HEK293T's exosome biogenesis speed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 201
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None