Part:BBa_K3848009
LLDR
LldR from Escherichia coli (E. coli) is a lactate-responsive transcription factor belonging to the GntR family. LldR governs the expression of the lldPRD operon in various bacterial species including E. coli and Corynebacterium glutamicum (BBa_K4584000).
LldR represses the expression of the lldPRD operon by binding to the O1 and O2 operator regions, in E. coli. In presence of L-lactate, the affinity of LldR to the operator regions changes, resulting in expression of lactate dehydrogenase and lactate permease.
Members of the GntR family are characterised by a highly conserved winged helix-turn-helix motif (wHTH) in their N-terminus domain (NTD) responsible for DNA binding. This helix-turn-helix motif is comprised of a tri-helical core, with helices 2 and 3 interacting specifically with the major groove of the DNA. Following the helical core, two beta strands are linked through a small loop, referred to as the "wing" motif. However, these transcription factors display variations in their C-terminal effector-binding and oligomerization domains. In the case of LldR, the C-terminal effector binding domain is responsible for binding to L-lactate.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 567
- 1000COMPATIBLE WITH RFC[1000]
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