Coding

Part:BBa_K4825024

Designed by: Cai Luxi   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 13:25, 11 October 2023 by Registry (Talk | contribs)

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pXyl

"pXyl is a synthetic inducible promoter that is regulated by inducer xylose. It is constructed to be the minimal inducible promoter in eukaryotes.

This part is sensitive to xylose and repressed by glucose, but insensitive to other metabolites. It is associated with the xylose-dependent activator XylR. The galactose inductibility of this part has been widely used in heterologous protein expression. In our project, this part is utilized to construct a plasmid with Bax gene to fulfill the kill switch design with a logic gate induced by xylose in S.cerevisiae. "

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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