Coding

Part:BBa_K4759065

Designed by: Junyuan Shi   Group: iGEM23_Jiangnan-China   (2023-10-02)
Revision as of 07:12, 2 October 2023 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


petH-RBS2-petF(Q62E)

The P450 enzymes are redox-dependent proteins, through which they source electrons from reducing cofactors to drive their activities. This part is coding for ferredoxin reductase PetH (SEQ ID NO.7) and ferredoxin PetF (SEQ ID NO.8) from the algae (Synechocystis PCC 6803) as the redox chaperones of OleP. We construct the plasmid pRSFDuet-petH-petF(Q62E)-olep to express PetH, PetF and Olep in E. coli C43.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1249
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1249
    Illegal NotI site found at 1022
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1249
    Illegal BglII site found at 1560
    Illegal BamHI site found at 1243
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1249
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1249
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None