Coding

Part:BBa_K4825005

Designed by: Cai Luxi   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 12:38, 11 October 2023 by Registry (Talk | contribs)

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SC Ma

"ScMα is the native signal peptide in S.cerevisiae that can target heterologous proteins to the extracellular environment of yeast cells.

This part as a signal peptide can enhance the ability of S.cerevisiae in secreting heterologous or recombinant enzymes by determining and trafficing the secretion pathway of target genes, but yet the secretion efficiency is not efficient enough.

In our project, this part is used to secrete heterologous protein Cry1518-35 out of S.cerevisiae, of which the efficiency is examined by secreting red fluorescence protein (RFP). This part can be applied as a robust control for heterologous expression in S.cerevisiae."

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None