![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4825005
Designed by: Cai Luxi Group: iGEM23_GreatBay-SCIE (2023-10-11)
SC Ma
"ScMα is the native signal peptide in S.cerevisiae that can target heterologous proteins to the extracellular environment of yeast cells.
This part as a signal peptide can enhance the ability of S.cerevisiae in secreting heterologous or recombinant enzymes by determining and trafficing the secretion pathway of target genes, but yet the secretion efficiency is not efficient enough.
In our project, this part is used to secrete heterologous protein Cry1518-35 out of S.cerevisiae, of which the efficiency is examined by secreting red fluorescence protein (RFP). This part can be applied as a robust control for heterologous expression in S.cerevisiae."
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |