Part:BBa_K3384313
pprm1 Pro
Multiple copies of putative Ste12 binding sites are prevalent in pheromone-responsive promoters of Saccharomyces cerevisiae, which are also known as pheromone response elements (PREs). pprm1 contains 3 × PREs which is in the opposite direction of the promoter. pprm1 Pro is a modified pheromone-responsive promoter. It contains 3 × PREs in the same direction of the promoter.
Characterization
When assembled with promotor BBa_K3384314, BBa_K3112009, and BBa_K3384311 in pRS415, this construct expressed GFP. Then the activity of these promoters can be quantitatively measured through flow cytometer. As is shown in figure 1, the pheromone concentration has no significant effect on the GFP expression intensity under the control of pprm1 Pro, indicating that this engineered pprm1 remains a stable expression level when induced by pheromone. With a low and stable induced expression level, this part assembled with reporter genes, for example, chromoproteins, can be used in co-expression scenarios with some colored products, and its weak expression intensity will not affect the observation of its colored products.
Varistor
The fluorescence expression intensity of BY4741 pRS415-prm1 Pro-GFP-CYC1 BBa_K3384313
https://static.igem.wiki/teams/4779/wiki/nanjing-bioxstem-pprm1-pro.png
===Reference===
1. LSengupta, P., and Cochran, B. H. (1990) The Pre and Pq Box Are Functionally Distinct Yeast Pheromone Response Elements, Molecular and Cellular Biology 10, 6809-6812.
2Hagen, D. C.; McCaffrey, G.; Sprague, G. F., Jr., Pheromone response elements are necessary and sufficient for basal and pheromone-induced transcription of the FUS1 gene of Saccharomyces cerevisiae. Mol Cell Biol 1991, 11 (6), 2952-61.
3Liu, Y.; Huang, Y.; Lu, R.; Xin, F.; Liu, G., Synthetic biology applications of the yeast mating signal pathway. Trends Biotechnol 2022, 40 (5), 620-631.
Sequence and Features
| chassis | Saccharomyces cerevisiae |