Composite

Part:BBa_K4604018

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-07)
Revision as of 17:35, 11 October 2023 by NikitaEdel (Talk | contribs)


piG_02b (tetR_riboK12_mazF_mTurq)

BioBrick piG_02b is a plasmid consisting of the tet promoter/repressor, an AdoCbl riboswitch, MazF fused together with mTurquoise and the rrnB terminator. The backbone we used in the experiments is pGGAselect. The tet promoter is a BioBrick from iGEM Freiburg 2022 (BBa_K4229059). This is an improved version of BioBrick BBa_K4604017.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 710
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 883
    Illegal AgeI site found at 1376
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2250


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Categories
Parameters
None