Coding

Part:BBa_K4830029:Design

Designed by: Ong Jie Hao   Group: iGEM23_NTU-Singapore   (2023-10-11)
Revision as of 17:49, 11 October 2023 by Registry (Talk | contribs)

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eGFP_complex premature stop codon


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 192
    Illegal PstI site found at 160
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 192
    Illegal PstI site found at 160
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 192
    Illegal PstI site found at 160
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 192
    Illegal PstI site found at 160
    Illegal NgoMIV site found at 734
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The eGFP_complex premature stop codon requires a specific mutation to the wild-type eGFP.


Source

The eGFP_complex premature stop codon is derived from the wild-type eGFP. The wild-type eGFP is derived from Aequorea victoria. It has an excitation maxima of 488 nm and emission maxima of 509 nm.

References