Coding

Part:BBa_K4830029

Designed by: Ong Jie Hao   Group: iGEM23_NTU-Singapore   (2023-10-11)
Revision as of 17:49, 11 October 2023 by Registry (Talk | contribs)

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eGFP_complex premature stop codon

The eGFP_complex premature stop codon is a derivative of the eGFP_simple premature stop codon. Similar to the eGFP_simple premature stop codon, the eGFP_complex premature stop codon can be utilized in a Traffic Light Reporter (TLR) assay.

However, the edit required is complex as it involves the truncation in a particular region from 42bp to 31bp.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 192
    Illegal PstI site found at 160
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 192
    Illegal PstI site found at 160
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 192
    Illegal PstI site found at 160
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 192
    Illegal PstI site found at 160
    Illegal NgoMIV site found at 734
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/gfp
Parameters
colorGreen
emission509nm
excitation488nm