Part:BBa_K283002

Speed control device (pLacI-rbs-TetR-terminator-ptet-rbs-chez_his-double terminators)

This is a device that enable the control of chez(histag) using anhydrotetracycline or tetracycline. The pLacI promoter(R0010)enables high level of expression of the TetR repressor(C0040) protein.Without the presence of LacI repressor molecule, the pLacI promoter is constitutively on to express the TetR repressor protein.Therefore the ptet(R0040) promoter is repressed under normal condition. However, in the presence of anhydrotetracycline or tetracycline, which binds to TetR and remove its inhibiting effect on pTet promoter, the chez-histag(K283008) protein can be expressed. Consequently, the expression level of chez-histag,which responsible for the dephosphorylation of cheY protein in bacteria flagella movement, should be controlled by the concentration of anhydrotetracycline or tetracycline.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1782
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]