Regulatory
pArsR

Part:BBa_K190015:Design

Designed by: Michael Verhoeven   Group: iGEM09_Groningen   (2009-07-15)
Revision as of 04:45, 20 October 2009 by Verhoeven1981 (Talk | contribs) (→‎References)

Arsenic Promoter (ArsR regulated)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The ArsR regulated promoter has been cloned into the BBa_J61002-R0040 plasmid for construction of promoter basic parts and their derivatives.

Insertion of a promoter element between the XbaI and SpeI sites resulted in a RFP reporter while retaining the ability to do biobrick assembly.

PBca1020-r0040.jpg

Source

E.coli TOP10

References

1) C. Xu, W. Shi, and B.P. Rosen (1996) The Chromosomal arsR Gene of Escherichia coli Encodes a trans-acting Metalloregulatory Protein, The Journal of Biological Chemistry, Vol. 271, No. 5, Issue of February 2, pp. 2427–2432.