Translational_Unit

Part:BBa_K4586031

Designed by: Mohamed Mohamed Saad Aboelghare   Group: iGEM23_AFCM-Egypt   (2023-10-04)
Revision as of 17:01, 11 October 2023 by Mohamed-Ab0elghare (Talk | contribs) (→‎Usage and Description)


ZF21.16 minCMV promoter - Cre recombinase

Usage and Description

This composite part is a translational unit implemented in our project for conditional expression of Cre recombinase enzyme following stimulation of vimentin syn notch receptor. This enzyme controls the conditional expression of our booster genes (SDC4, STEAP3, NadB) (BBa_K4586027) by deleting (STOP) sequence flanked by two loxP sites upstream to the coding of our booster genes.

This figure illustrates the role of the Cre loxP system in regulation the expression of our booster genes.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 673


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Categories
Parameters
None