![](https://parts.igem.org/images/partbypart/icon_plasmid_backbone.png)
Plasmid_Backbone
Part:BBa_K228867
Designed by: Lin Min Group: iGEM09_PKU_Beijing (2009-09-22)
pSB1A2-lacZ screening plasmid
T7 polymerase with an amber mutation coding gene is constructed after AraC protein(reversed sequence) and Pbad promoter which would be triggered by Arabinose. T7 polymerase can work well in the exsitence of SupD tRNA. Besides, the expression of the T7 polymerase can be regulated by RBS.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a suffix.
Illegal SpeI site found at 2
Illegal PstI site found at 303 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2345
Illegal NheI site found at 14
Illegal NheI site found at 37
Illegal SpeI site found at 2
Illegal PstI site found at 303
Illegal NotI site found at 2351 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2345 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 2345
Plasmid lacks a suffix.
Illegal SpeI site found at 2
Illegal PstI site found at 303 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2345
Plasmid lacks a suffix.
Illegal XbaI site found at 2360
Illegal SpeI site found at 2
Illegal PstI site found at 303 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 1384
[edit]
Categories
Parameters
n/a | pSB1A2-lacZ screening plasmid |