RNA

Part:BBa_K4830019

Designed by: Ong Jie Hao   Group: iGEM23_NTU-Singapore   (2023-10-11)
Revision as of 14:29, 11 October 2023 by Jong154 (Talk | contribs)


TLR pegRNA 1

TLR pegRNA 1 - prime editing guide RNA (pegRNA) targeting the premature stop codon instilled in a mCherry fluorescent protein (mCherry); introduces T to G (A to C) nucleotide change. The premature stop codon is edited into the translatable amino acid - glycine, restoring the wild-type mCherry sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/rfp
//classic/reporter
Parameters
biologyHuman
colorRed