Composite

Part:BBa_K4757999:Design

Designed by: Marik Müller   Group: iGEM23_Heidelberg   (2023-10-07)
Revision as of 12:50, 7 October 2023 by Registry (Talk | contribs)

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Synthetic expression cassette regulated by terepthalic acid and alkanes for PET and PE sensing


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4202
    Illegal EcoRI site found at 4298
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4202
    Illegal EcoRI site found at 4298
    Illegal NotI site found at 1625
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4202
    Illegal EcoRI site found at 4298
    Illegal BglII site found at 212
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4202
    Illegal EcoRI site found at 4298
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4202
    Illegal EcoRI site found at 4298
    Illegal NgoMIV site found at 934
    Illegal NgoMIV site found at 2716
    Illegal NgoMIV site found at 3187
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

XylS/Pm, AlkS/pAlkB is natively found in Pseudomonas putida, sRNA scaffold (SgrS) is natively found in Escherichia coli, mKate2 originiated from Entacmaea quadricolor. All sequences, except XylS/Pm were obtained by gene synthesis

References