Composite

Part:BBa_K4583053

Designed by: Suiru Lu   Group: iGEM23_SDU-CHINA   (2023-10-05)
Revision as of 13:38, 11 October 2023 by Lusuiru (Talk | contribs) (→‎Usage and Biology)


PHB production Regulation: PesaS-B0034-gltA-PesaRwt-B0034-PHBcab-PYU16-B0034-SRRz

PesaS-B0034-gltA-PesaRwt-B0034-PHBcab-PYU16-B0034-SRRz

Usage and Biology

Many production processes using microorganisms face the dilemma of conflicting production products and key metabolic pathways. This means that simply introducing product-synthesising genes into engineered bacteria can greatly affect the growth of the microorganism, leading to a situation where production is too low. There are a number of current solutions to this problem. For example, metabolic engineering can regulate metabolic flow using methods such as gene knockdown, promoter replacement, etc. These static strategies are effective for productivity improvement, but are not responsive to changes in the cell or environment.

This part is about PHB Production Regulation based on an three-layer dynamic regulation model. It successfully regulate the PHB production by dividing the process into 3 phases: Growth Phase, Production Phase and Product-release Phase. In this way, the cell factory can firstly grow

1. Three-layer Dynamic Regulation Model

2. The first layer--Growth control

3. The second layer--Production control

4. The Third layer--Product-release control

Characterization

1. Protocols

2. The Characterization of PesaS and PesaRwt/PesaRc/PesaRp

3. The Characterization of PesaRwt/PesaRc/PesaRp and PYU3/PYU7/PYU16/PYU92

Summary of Results

Our part provides an example for future iGEM team

Limitations

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2740
    Illegal BglII site found at 3565
    Illegal BamHI site found at 1791
    Illegal XhoI site found at 1511
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2046
    Illegal NgoMIV site found at 2117
    Illegal NgoMIV site found at 2717
    Illegal NgoMIV site found at 3029
    Illegal NgoMIV site found at 3308
    Illegal NgoMIV site found at 3960
    Illegal NgoMIV site found at 3982
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5826
    Illegal SapI.rc site found at 769


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Categories
Parameters
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