Translational_Unit

Part:BBa_K4879009:Design

Designed by: Anuvind Pramod   Group: iGEM23_IISER-Pune-India   (2023-10-08)
Revision as of 10:20, 8 October 2023 by Registry (Talk | contribs)

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Yarrowia lipolytica HygR construct


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1561
    Illegal suffix found in sequence at 1831
    Illegal EcoRI site found at 684
    Illegal EcoRI site found at 778
    Illegal EcoRI site found at 1825
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 684
    Illegal EcoRI site found at 778
    Illegal EcoRI site found at 1561
    Illegal EcoRI site found at 1825
    Illegal SpeI site found at 1832
    Illegal PstI site found at 1846
    Illegal NotI site found at 1567
    Illegal NotI site found at 1839
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 684
    Illegal EcoRI site found at 778
    Illegal EcoRI site found at 1561
    Illegal EcoRI site found at 1825
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1561
    Illegal suffix found in sequence at 1832
    Illegal EcoRI site found at 684
    Illegal EcoRI site found at 778
    Illegal EcoRI site found at 1825
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1561
    Illegal EcoRI site found at 684
    Illegal EcoRI site found at 778
    Illegal EcoRI site found at 1825
    Illegal XbaI site found at 1576
    Illegal SpeI site found at 1832
    Illegal PstI site found at 1846
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

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References