Composite

Part:BBa_K4779003:Design

Designed by: Ziqi Mi   Group: iGEM23_Nanjing-BioXstem   (2023-09-26)
Revision as of 13:30, 26 September 2023 by Registry (Talk | contribs)

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CMPS Pro:A yeast copper-induced reporter pathway with Positive feedback loop with prm1pro promoter


Assembly Compatibility:
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    INCOMPATIBLE WITH RFC[10]
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    INCOMPATIBLE WITH RFC[21]
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    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3138
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    Illegal SpeI site found at 523
    Illegal SpeI site found at 4393
    Illegal SpeI site found at 4939
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    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3138
    Illegal XbaI site found at 279
    Illegal XbaI site found at 517
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    Illegal SpeI site found at 523
    Illegal SpeI site found at 4393
    Illegal SpeI site found at 4939
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    INCOMPATIBLE WITH RFC[1000]
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Design Notes

When designing the sequence in detail, one of the key considerations is how to convert copper ion signals into pheromone signals more efficiently and sensitively. Drawing from the insights of the second DBTL, specifically in the context of CMPS (BBa_K4779002) with its incorporated positive feedback loop utilizing Ste5ΔN-CTM, we replaced the original pprm1 with the more sensitive pprm1 Pro promoter for GFP expression. This strategic modification significantly amplifies the sensitivity in detecting pheromone signals, resulting in the creation of CMPS Pro (BBa_K4779003). This is the third round DBTL.


Source

CMPSpro is a new composite part (BBa_K4779003). We obtained the gene sequences of the promoter of Cup1 (BBa_K945002), MFα2 (BBa_K110005), the promoter of prm1(BBa_K1346004), the promoter of prm1 pro(BBa_K3384313)，green fluorescent protein (GFP) (BBa_K2176000), Ste5ΔN-CTM(BBa_K3384315) and the terminator CYC1(BBa_K4278703) from IGEM, and sent them to GENEWIZ (Suzhou Genewiz Biotechnology Co., Ltd.) for synthesis.

References