Part:BBa_K4623003

TrwC, a protein linker to covalently link ssDNA 5' end

TrwC is a type of relaxase, which participates in bacterial DNA binding and horizontal transfer of genomic material between bacteria​ [1]​. In vitro, it can specifically recognize the nic sequence (GGTGCGTATTGTCT^ATAGC) on ssDNA, causing it to break, and covalently bind to the ssDNA.

Therefore, TrwC relaxase possesses sequence specificity, orthogonality, and the ability to form a covalent bond with standard oligonucleotides. This provides a potential method in our project design that may be superior to existing protein-bound DNA nanostructures. The linkage of TrwC with ssDNA in vitro depends on Mg2+, so the reaction can be initiated by adding Mg2+ in the mixed system. Conversely, adding EDTA can terminate the reaction and yield DNA-protein complexes [​2]​.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 286
  Illegal PstI site found at 604
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 286
  Illegal PstI site found at 604
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 286
  Illegal PstI site found at 604
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 286
  Illegal PstI site found at 604
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 409
  Illegal SapI.rc site found at 124