![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4800054
Designed by: Chenxi Ma Group: iGEM23_NJTech-China-B (2023-10-09)
pTrc99a-davB-davA-GabT
davB-davA-GabT gene encodes for carboxylate reductase, alcohol dehydrogenase and transaminase. These enzymes can realize the metabolic pathway from lysine to glutarate semialdehyde, and the three enzymes form a synthetic module including 5-HV.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 5122
Illegal XbaI site found at 6489
Illegal PstI site found at 6501 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 5122
Illegal PstI site found at 6501
Illegal NotI site found at 2808 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 5122
Illegal BglII site found at 3897 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 5122
Illegal XbaI site found at 6489
Illegal PstI site found at 6501 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 5122
Illegal XbaI site found at 6489
Illegal PstI site found at 6501
Illegal NgoMIV site found at 4057
Illegal AgeI site found at 3464
Illegal AgeI site found at 3713
Illegal AgeI site found at 5535 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 6685
Illegal BsaI site found at 7753
Illegal SapI.rc site found at 767
[edit]
Categories
Parameters
None |